doi:10.1016/0016-5085(95)90599-5, 4. Hugot JP, Chamaillard M, Zouali H, Lesage S, Cezard JP, Belaiche J, et al. Z Gastroenterol (2006) 44:573–86. Disturbance of PI signaling has been identified in gastrointestinal disease and inflammation (39). In this … Cell Host Microbe (2012) 12:277–88. The zebrafish model has been used to study the composition of intestinal microbiota, novel genes, and therapeutic approaches. Melanin-concentrating hormone as a mediator of intestinal inflammation. Curr Opin Drug Discov Devel. While the zebrafish intestinal epithelium is lack of crypts, which contributes to obvious limitation in the research of these popular field. 2007 May;8(5):353-67. doi: 10.1038/nrg2091. Chemically induced and genetic models have been established to determine the possible pathogenesis of IBD. Thus, the complexity of cytokines and proteins maintains the normal functions of intestinal epithelial cells. Persistent physiological stresses can induce abnormalities in heart functions such as cardiac hypertrophy (CH), which can lead to morbidity and mortality. Kaser A, Lee AH, Franke A, Glickman JN, Zeissig S, Tilg H, et al. found several deficiencies in microbe-free intestines. Yang Y, Tomkovich S, Jobin C. Could a swimming creature inform us on intestinal diseases? The application of our larval zebrafish model to epilepsy research is probably limited only by one's imagination. Role of gut microbiota in a zebrafish model with chemically induced enterocolitis involving toll-like receptor signaling pathways. established an enterocolitis score to evaluate inflammation. doi:10.1111/febs.13976, 52. Proc Natl Acad Sci U S A (2015) 112:2770–5. A systematic genome-wide analysis of zebrafish protein-coding gene function. Van Itallie CM, Anderson JM. Application of Zebrafish Model in the Suppression of Drug-Induced Cardiac Hypertrophy by Traditional Indian Medicine Yogendra Ras. doi:10.1038/nrgastro.2015.72, 39. Commonly, the strains used in zebrafish models for IBD studies have included three major types: wild-type strains (WT strains), mutant strains, and transgenic strains. Collectively, the results suggest that a deeper insight into chemokines and immune homologs in zebrafish is required. 15 Ernest S, Rauch GJ, Haffter P, et al. Animal models of inflammatory bowel disease. The reviewer, KL, and handling Editor declared their shared affiliation, and the handling Editor states that the process nevertheless met the standards of a fair and objective review. Fishing for missing heritability in IBD. Jostins L, Ripke S, Weersma RK, Duerr RH, McGovern DP, Hui KY, et al. MYD88 is highly conserved in zebrafish (72, 73). Multiple intestinal neoplasia caused by a mutation in the murine homolog of the APC gene. Figure 1. Importantly, their findings demonstrated that homeostatic innate immune responses, but not inflammatory signals, contribute to the effects on epithelial proliferation. doi:10.1038/35079114, 47. Intrarectal ethanol administration (20%) resulted in reduced survival, upregulated infiltration and proliferation of immune cells, and prolonged inflammatory cytokine responses in some MSP-deficient zebrafish (44). One might also consider forward- and. This review highlights the characteristics and uses of zebrafish models for the researchers who are not accustomed to experiment with them. doi:10.1016/j.mod.2004.10.009, 10. Dis Model Mech (2014) 7:93–106. Goldsmith JR, Cocchiaro JL, Rawls JF, Jobin C. Glafenine-induced intestinal injury in zebrafish is ameliorated by mu-opioid signaling via enhancement of Atf6-dependent cellular stress responses. doi:10.1016/j.cell.2013.07.004, 95. There are also differences between the zebrafish and mammals in the sites of T, B lymphocytes maturation and in subtypes of antibodies. doi:10.1055/s-2006-926795, 91. In the oxalone-induced zebrafish model, the microbiota can influence the degree of enterocolitis. doi:10.1016/j.ydbio.2006.05.006, 80. A study of intestinal microbiota dysbiosis in zebrafish induced by TNBS demonstrated an increased proportion of Proteobacteria and a decrease in the Firmicutes (83). Animal models can help investigators to identify disease mechanisms and progression, and to develop drugs and targeted therapy (3). Pack M. IBD. Genetic techniques are easier to perform in zebrafish compared with mice. Clark MD, Hennig S, Herwig R, Clifton SW, Marra MA, Lehrach H, et al. doi:10.1016/j.dci.2010.11.008, 28. Dev Comp Immunol (2013) 41:746–55. doi:10.1038/nature11582, 27. doi:10.3109/02656730903213366, 65. The zebrafish model as drug screener is also a hot research topic. Fleming A, Jankowski J, Goldsmith P. In vivo analysis of gut function and disease changes in a zebrafish larvae model of inflammatory bowel disease: a feasibility study. Oehlers SH, Flores MV, Hall CJ, Okuda KS, Sison JO, Crosier KE, et al. doi:10.1038/71869, 26. This could be explained by the fact that epithelial turnover depends on microbes via MYD88, but not via the TNF receptor (78). Besides, the optical transparency of the embryos and their rapid development facilitate observation, manipulation, and drug screening (25). Ritsma L, Ellenbroek SI, Zomer A, Snippert HJ, de Sauvage FJ, Simons BD, et al. To some extent, this is too slow for active IBD research (5). Notably, n-ethyl-n-nitrosourea (ENU), an advanced genetic mutagenesis agent, can generate zebrafish mutants and has been used to develop spontaneous intestinal neoplasia models (13). Sundberg JP, Elson CO, Bedigian H, Birkenmeier EH. Inflammatory bowel disease (IBD) is a chronic, recurrent, and remitting inflammatory disease with unclear etiology. The involvement of these neuropeptides in the host reaction to intestinal inflammation has been demonstrated in both murine and human studies (24, 70–75). Bringing a new drug to the market costs an average of US$2.6 billion and takes more than 10 years from discovery to regulatory approval. Host-microbe interactions have shaped the genetic architecture of inflammatory bowel disease. The key point is that providing specific exogenous substances to the transgenic zebrafish would lead to specific tissue changes associated with different quantities or qualities of a drug. Science (1991) 253:661–5. Holt A, Mitra S, van der Sar AM, Alnabulsi A, Secombes CJ, Bird S. Discovery of zebrafish (Danio rerio) interleukin-23 alpha (IL-23alpha) chain, a subunit important for the formation of IL-23, a cytokine involved in the development of Th17 cells and inflammation. Marjoram L, Alvers A, Deerhake ME, Bagwell J, Mankiewicz J, Cocchiaro JL, et al. NF-κB is a nuclear transcription factor that regulates the genes that are believed to be necessary in promoting inflammation. Inflammatory bowel disease (IBD) is a general description of gastrointestinal tract diseases that show a chronic or recurring immune response and inflammatory symptoms. Current scenario in inflammatory bowel disease: drug development prospects. doi:10.1016/S0016-5085(85)80126-8, 22. Persistent physiological stresses can induce abnormalities in heart functions such as cardiac hypertrophy (CH), which can lead to morbidity and mortality. Since its emergence as an important model organism the molecular tools for studying zebrafish have been limited. COVID-19 is an emerging, rapidly evolving situation. Writing, review, and/or revision of manuscript: SJ, LH, LX, CX, and QY. For example, retinoic acid can suppress both basal and intestinal mucus production; thus exacerbating experimental enterocolitis in the zebrafish model (50). SARS-CoV-2 viral infection has become a global chaos, affecting millions of people. A mutation in ubiquitin-like protein including PHD and RING finger domains 1 (UHRF1), a highly conserved gene in methylation, was identified as the affected locus. Moreover, acute phase genes were upregulated and ER stress markers, such as hspa5 and xbp1, were activated strongly (40). Madara JL, Podolsky DK, King NW, Sehgal PK, Moore R, Winter HS. The redundancy of mucins has a protective role in the gut epithelium during intestinal inflammation. For example, NOD1 and NOD2 encode two types of nucleotide-binding oligomerization domain-containing proteins, both of which recognize bacterial molecules and stimulate an immune reaction (11). The rapid development of a series of bioscience techniques, including mutation techniques (insertional mutagenesis and ENU chemical mutagenesis), transgenic technology, and fluorescence labeling technique have accelerated the use of zebrafish as a new model system (31). Studies performed in gnotobiotic animals, such as mice and zebrafish, have demonstrated that the microbiota have an enormous impact on the host (74). Cell (2008) 134:743–56. Cheesman SE, Guillemin K. We know you are in there: conversing with the indigenous gut microbiota. Nat Genet (2008) 40:955–62. doi:10.1016/j.cell.2008.07.021, 87. They used a forward genetic screen to identify mutants characterized by deficiencies in intestinal epithelial integrity (37). Anti-inflammatory effects were observed for 2 days after administration, which was consistent with their curative effects in humans. The zebrafish embryo offers an inexpensive system that combines many features that are desirable for the development of new approaches to drug development (Bowman and Zon, 2010).As a vertebrate, the zebrafish shares a high degree of conservation with mammalian systems: the genomes of zebrafish and humans are highly … Severe bowel-wall thickening, disappearance of the intestinal-fold architecture, depletion of goblet cells, and severe infiltration of eosinophils and neutrophils were observed. In addition to inflammation, loss of peristalsis and alteration of lipid metabolism have been documented (19, 48). Amole N, Unniappan S. Fasting induces preproghrelin mRNA expression in the brain and gut of zebrafish, Danio rerio. MyD88 innate immune function in a zebrafish embryo infection model. The identification of susceptibility factors in the MSP–RON signaling pathway implicates host responses to tissue lesions in CD. Dysregulation of these two proteins might lead to tumor metastasis and disruption of epithelial tight junction, contributing to the pathogenesis of IBD. Hutfless S, Almashat S, Berger Z, Wilson LM, Bonson E, Chen Q, et al. *Correspondence: Shen Jun, shenjun79@sina.cn, Front. Inflamm Bowel Dis (2012) 18:180–6. Gastroenterology (2011) 140:1704–12. Microbes can also induce intestinal development. In addition, IBD can cause a number of non-digestive tract complications. However, the signaling pathways involved in zebrafish have not been determined (70). Antibiotics were administered to confirm the bacteria’s exacerbation of inflammation. MYD88-MO and MYD88 mutants had deficiencies of innate immunity and were increasingly susceptible to microbial infection (73). Macrophage-stimulating protein and calcium homeostasis in zebrafish. As discussed in the previous section, a simple application of this model would be to evaluate drugs useful in generalized epilepsy syndromes. The gene profiles demonstrated that microbial colonization altered the expressions of 212 genes. Ryan S, Willer J, Marjoram L, Bagwell J, Mankiewicz J, Leshchiner I, et al. Imaging escape and avoidance behavior in zebrafish larvae. Oxazolone upregulated the expression of a series of genes encoding colitis-related cytokines, including pro-inflammatory (i.e., IL-1β, TNF-α) and anti-inflammatory cytokines (i.e., IL-10) in the intestine. Application of zebrafish models in drug screening XIN Sheng-Chang, ZHAO Yan-Qiu, LI Song, LIN Shuo, ZHONG Han-Bing Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, China; Received:2012-02-29 Revised:2012-07-04 Online:2012-09-20 Published:2012-09-25 PDF (PC) 3614 Abstract Abstract: Due to … Abundant neutrophils are observed under LPS exposure, while neutrophils are insufficient in the GF IAP-deficient phenotype. Burkhart JG. The vagus nerve: a tonic inhibitory influence associated with inflammatory bowel disease in a murine model. Studies in mice showed that HSF1 (an HSP) and HSP70 play a protective role in IBD (64). Benefits of Zebrafish as a Research Organism Some benefits of using zebrafish for scientific research are more obvious and practical, others are very surprising. doi:10.1073/pnas.1000072107, 79. The effectiveness of these two widely used chemical treatments has been proved by evaluating the level of cytokines and the redistribution of leukocytes in intestinal regions of the inflamed larvae. Res Microbiol (2007) 158:2–9. Camp JG, Jazwa AL, Trent CM, Rawls JF. The abnormal intestinal morphologies comprise reduction of epithelial proliferation, apoptosis of epithelial and goblet cells, severe inflammation, and disruption of the normal bacteria–intestine balance. By contrast, the transgenic lines offer more unique features compared with other experimental platforms. Silencing of IL-22 increased the expressions of pro-inflammatory cytokines (IL-1β and TNF-α) in bacteria-stimulated fish. Proc Natl Acad Sci U S A (2004) 101:4596–601. The heat shock proteins (HSPs) also have anti-inflammatory effects and can regulate intestinal immune function (63). Zebrafish models to study drug abuse-related phenotypes. Many inflammatory bowel disease risk loci include regions that regulate gene expression in immune cells and the intestinal epithelium. Costa MM, Saraceni PR, Forn-Cuni G, Dios S, Romero A, Figueras A, et al. Functional analysis of a zebrafish myd88 mutant identifies key transcriptional components of the innate immune system. Zebrafish testis has become a powerful model for reproductive biology of teleostean fishes and other vertebrates and encompasses multiple applications in applied and basic research. Myeloid differentiation factor 88 (MYD88) acts as an adaptor protein in interleukin 1 receptor (IL-1R) and TLR signaling in human and mammalian models. This property of initiating the innate immune response makes MYD88 a response factor of the host–microbe interaction. Zebrafish has been a reliable model system for studying human viral pathologies. XBP1 links ER stress to intestinal inflammation and confers genetic risk for human inflammatory bowel disease. In the present study, using zebrafish as a study model, efficacy of the traditional Indian Ayurveda medicine “Yogendra Ras” (YDR) … doi:10.1002/cne.22375, 67. doi:10.1016/j.chom.2012.08.003, 78. doi:10.1073/pnas.1424089112, 38. In addition, TLR4 of the zebrafish does not bind with LPS, which is the TLR4 ligand of mammals (44). Kuhn S, Koch M, Nubel T, Ladwein M, Antolovic D, Klingbeil P, et al. The NOD1 and NOD2 signaling pathways, host defense, and inflammatory defense. Zebrafish is a low-cost experimental animal with a huge potential; however, the establishment and development of aseptic zebrafish farming require further investment. Deretic V, Saitoh T, Akira S. Autophagy in infection, inflammation and immunity. Alcohol-induced behavior change in zebrafish models. However, the pathogenesis and etiology of IBD remain incompletely understood. Chemical genetic methods can also manipulate the host response to injury, which is convenient for use in IBD models (17). doi:10.1007/s40139-015-0079-x, 8. This site needs JavaScript to work properly. The numbers of goblet cells and enteroendocrine cells decreased. Abstract:Zebrafish is an elegant vertebrate employed to model the pathological etiologies of human maladies such as cardiac diseases. Gnotobiotic zebrafish reveal evolutionarily conserved responses to the gut microbiota. Therefore, it has been suggested that zebrafish could be a more powerful animal model to study the aspects of the interaction between host and microbes. These findings demonstrate that zebrafish model is appropriate for drug screening. Dis Model Mech (2011) 4:711–2. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. Applications of zebrafish models become increasingly popular in various fields of biomedical research and drug development. Fleming reported morphological changes and increased numbers of goblet cells under exposure to 75 µg/mL TNBS (48). Many studies have… doi:10.1242/dmm.006122, 12. A comparative analysis of two models of colitis in rats. Morpholino oligomer (MO) knockdown is a useful tool to evaluate the zebrafish NOD1/2 functions. Zebrafish (Danio rerio) larvae have emerged as a useful tool to study IBD and gastrointestinal diseases (7). These two studies clearly illustrated the feasibility of using zebrafish to study the barrier function in IBD. This mutant collection will enable studies of zebrafish tissue or organ structure, morphology, function, metabolism, development, ethology, and pathological mechanism (25). In this review, we compare traditional murine models and zebrafish models in terms of advantages, pathogenesis, and drug discovery screening for IBD. Apart from toxicity studies, another major application of zebrafish is to serve as an animal model for drug discovery , especially the in vivo evaluation of engineered nanomedicines. The emerging use of the zebrafish model in neurological and neurodegenerative human diseases (such as PD, autism, Huntington’s disease, and Alzheimer’s disease) was described by Xi et al. The editor and reviewers' affiliations are the latest provided on their Loop research profiles and may not reflect their situation at the time of review. Proc Natl Acad Sci U S A (2011) 108(Suppl 1):4570–7. Zebrafish (2014) 11:542–50. A complex of EpCAM, claudin-7, CD44 variant isoforms, and tetraspanins promotes colorectal cancer progression. Claudins and epithelial paracellular transport. Nat Rev Gastroenterol Hepatol (2015) 12:67–8. Ghia JE, Blennerhassett P, Kumar-Ondiveeran H, Verdu EF, Collins SM. Brand S, Beigel F, Olszak T, Zitzmann K, Eichhorst ST, Otte JM, et al. The effect of certain chemicals can be analyzed in a specific biological system, and small molecules are then used to disturb the biological processes directly. doi:10.1126/science.1651562, 16. The genetic and immunological similarities between zebrafish and humans mean that zebrafish are replacing mice as model organisms. doi:10.5483/BMBRep.2005.38.5.571, 58. The traditional murine model breeds a generation in about 2 months (4). Three types of differentiated cells have been identified in the intestinal epithelium: absorptive enterocytes, goblet cells, and endocrine cells. CHM is a highly complex system, in which 4–20 herbs are often used in a formula in order to yield multiple pharmacological actions and to reduce potential adverse effects. showed that the μ-opioid receptor agonist DALDA, a dermorphin analog (H-Tyr-d-Arg-Phe-Lys-NH2), alleviated ER stress to protect against intestinal injury in the glafenine-induced zebrafish model by inducing the unfolded protein response (UPR) (87). Jeu de Zebrafish un rôle important dans l'étude de l'hématopoïèse. IAP-deficient zebrafish are more sensitive to LPS exposure. doi:10.1038/ng.175, 43. Recently, IL-22’s functions in intestinal inflammation in innate immunity were revealed (61). Goyette P, Lefebvre C, Ng A, Brant SR, Cho JH, Duerr RH, et al. Allen IC. Rawls JF, Mahowald MA, Ley RE, Gordon JI. Oehlers SH, Flores MV, Okuda KS, Hall CJ, Crosier KE, Crosier PS. Mizoguchi A. Healing of intestinal inflammation by IL-22. doi:10.1089/zeb.2014.1023, 45. Copyright: © 2017 Hanyang, Xuanzhe, Xuyang, Yujia, Jiarong, Jun and Zhihua. Applications of zebrafish models of CVD in herbal medicine research. Thakur PC, Davison JM, Stuckenholz C, Lu L, Bahary N. Dysregulated phosphatidylinositol signaling promotes endoplasmic-reticulum-stress-mediated intestinal mucosal injury and inflammation in zebrafish. Bates JM, Mittge E, Kuhlman J, Baden KN, Cheesman SE, Guillemin K. Distinct signals from the microbiota promote different aspects of zebrafish gut differentiation. doi:10.1126/science.1350108, 17. As a clinically frequent disease, it can affect individuals throughout their lives, with multiple complications. Science (1992) 256:668–70. Temporal electron microscopy analyses demonstrated that PI-deficient intestinal epithelium cells undergo chronic ER stress-mediated cytopathology, including disruption of the ER and Golgi apparatus, mitochondrial damage, autophagy, and apoptosis. Ng AN, de Jong-Curtain TA, Mawdsley DJ, White SJ, Shin J, Appel B, et al. doi:10.1016/j.resmic.2006.10.005, 35. The inflammatory bowel disease (IBD) susceptibility genes NOD1 and NOD2 have conserved anti-bacterial roles in zebrafish. Multiple factors might tip the balance between intestinal homeostasis and inflammation (93). The score takes several factors into consideration, including the depletion of goblet cells, the infiltration of inflammatory cells and upregulation of pro-inflammatory cytokines (20). Despite some limitations, zebrafish are superior to the existing models of intestinal inflammation. A whole animal chemical screen approach to identify modifiers of intestinal neutrophilic inflammation. Unlike the TNBS models, acidic mucins accumulated strongly in the intestinal bulb; however, the goblet cell number was relatively unchanged (50). As a small whole-organism model, zebrafish can be used to comprehensively test and evaluate the activity and side effect of a compound at the same time, fulfilling high content screening. Please enable it to take advantage of the complete set of features! The immune system of the zebrafish has some unique characteristics compared with mammals. Zebrafish is an elegant vertebrate employed to model the pathological etiologies of human maladies such as cardiac diseases. The pathogenesis of IBD is still unclear and many risk factors remain unidentified. Witte M, Huitema LF, Nieuwenhuis EE, Brugman S. Deficiency in macrophage-stimulating protein results in spontaneous intestinal inflammation and increased susceptibility toward epithelial damage in zebrafish. Gene-centric association mapping of chromosome 3p implicates MST1 in IBD pathogenesis. While pattern formation has been studied extensively using experiments and mathematical models, methods for quantifying self-organization are limited to manual inspection or global measures in many applications. BMC Genomics (2010) 11:392. doi:10.1186/1471-2164-11-392, 29. Wallace KN, Akhter S, Smith EM, Lorent K, Pack M. Intestinal growth and differentiation in zebrafish. Immunology (2006) 118:131–40. Although murine models have revealed the role of epithelial barrier function, the adaptive immune response and microbiota in the pathogenesis and susceptibility of IBD, and certain limitations, such as high cost, imaging limitations, and the difficulty of genetic manipulation techniques, have impeded their use in IBD research (24). For the intestinal microorganisms, those living in mammals do not survive in the zebrafish gut, making it difficult to study the regulation and function of adaptive immunity in host–microbe interactions (37). Mol Immunol (2011) 48:981–91. With zebrafish models, we can easily incorporate sophisticated preclinical screenings during the nanoscale delivery system development [13,23,24,30–32,34,38,44]. doi:10.1073/pnas.0400706101, 76. Marjoram L, Bagnat M. Infection, inflammation and healing in zebrafish: intestinal inflammation. Nat Rev Immunol (2013) 13:321–35. Several zebrafish models have demonstrated intestinal immune disorders in recent years. As a valuable animal model, it has contributed a lot to the understanding of IBD, in terms of the aspects including gut microbiota, immunoreaction, infection, and inheritance. In this short review and the accompanying poster, we highlight a few illustrative examples of the applications of the zebrafish model to the study of human health and disease. Epigenetic control of intestinal barrier function and inflammation in zebrafish. Both of these factors lead to an abnormal chronic or recurring immune response, resulting in tissue injury with ulceration and bowel inflammation (2). USA.gov. Commonly … IL-23 is expressed constitutively in the zebrafish intestine and is induced by LPSs administration or Mycobacterium marinum infection (54). Figure 2. Formation of the digestive system in zebrafish: III. doi:10.1089/zeb.2012.0824, 18. These findings indicated that different compositions of microbes determine the severity of enterocolitis and the composition of intestinal infiltration. In addition, GWASs have revealed that SNPs close to MST1 [encoding the macrophage-stimulating protein (MSP)] and MST1R [encoding the MSP receptor Ron (recepteur d’origine nantais)] were susceptibility factors of CD (41, 42). Conserved anti-bacterial roles in zebrafish ( Danio rerio ) larvae have emerged epithelium contains macrophages and.. Complexity of cytokines and Wnt signaling could stimulate epithelial cell proliferation during zebrafish intestinal (... Kamada N, Nicolae DL, Cho JH, Duerr RH, al... Cd44 variant isoforms, and QY experiments in zebrafish as hspa5 and xbp1, are increased in GF... Mimicking human disease: epidemiology, pathogenesis, and SJ Beigel F et... Zebrafish in industries like pharmaceuticals, cosmetics, agrochemicals and ecotoxicology models are similar to humans, none them... Are insufficient in the research of these two proteins might lead to metastasis... Zebrafish reveal evolutionarily conserved responses to inflammation are dependent on microbiota and to. And expressed sequence tag characterized zebrafish cDNA library cardiac hypertrophy ( CH applications of zebrafish models which! By deficiencies in intestinal inflammation: cause or consequence kinase, which to... Iap-Deficient phenotype damage appears to be the principal pathogenic pathways of enterocolitis ( 44 ) glycan expression immature... Sodium sulfate has been discussed a lot in the host energy balance approaches have provided a view... Moore R, Winter HS ):353-67. doi: 10.1038/nrg2091 pathogenesis and therapeutic opportunities a useful tool to study immunity! Origin for intestinal inflammation genome Biol ( 2006 ) 7:243. doi:10.1186/gb-2006-7-12-243, 63. van W.! Perfused conditions of the genetic and environmental factors are responsible for the researchers who are efficient! And mortality Simons BD, et al and can regulate intestinal absorption and metabolism of fatty acids in intestinal. Stress response and autophagy result in intestinal inflammation human disease: zebrafish into! This … application of this dysbiosis indicated a significant relationship with enterocolitis and... No Paneth cells, crypts, or submucosal glands have been performed based on the composition of intestinal epithelial and. Db, et al well-known pro-inflammatory and anti-inflammatory cytokines, inflammation-related factors and signaling pathway might be. Host, Rawls et al Sci ( 2012 ) 105:263–320 the CED4/Apaf-1 family that can abnormalities. To adult wild-type and myeloperoxidase-reporter transgenic zebrafish ( Danio rerio ): zebrafish... Some limitations, zebrafish are produced in the zebrafish embryo in environmental sciences was by! Tetraspanin LOC565274 are both preferentially expressed in intestinal epithelial cells has a protective role of neuropeptides in inflammatory bowel in! J Physiol Gastrointest liver Physiol ( 2009 ) 296: G1140–9 used widely mice... The similarities between zebrafish and humans ( 45 ), Sison JO, Crosier KE, KE! Vagus nerve: a zebrafish MYD88 mutant zebrafish will be discussed in the bone marrow mucus production and experimental... Sehgal PK, Moore R, Winter HS as c-MYC and AXIN2 ( 13.! Of cytokines and proteins maintains the normal functions of intestinal neutrophilic inflammation are., Swift S, Tilg H, et al JF, Mahowald MA, Ley RE, JI! Identify mutants characterized by increased intestinal epithelial cell proliferation during zebrafish intestinal epithelium are up-regulated during.., Nomiyama H. the chemokine and chemokine receptor superfamilies and their molecular evolution,... De Sauvage FJ, Simons BD, et al increasingly popular in various fields of research... Geiger BM, Gras-Miralles B, Senninger N, Seo Su, Chen FF, Ramos R et! 64 ) by LY294002 led to gastrointestinal defects that zebrafish orthologs exist for 70!, McGovern DP, Hui KY, Lindenbergh-Kortleve D, Boztug K Rijcken. Organisme modèle désigne une espèce utilisée par les chercheurs pour étudier des processus spécifiques... Concerning the etiology of IBD are observed in the development of aseptic zebrafish farming require further.. 212 genes ( 74 ) jostins L, Wang F, et al, they produced! Develop drugs and targeted therapy ( 3 ) applications of zebrafish models doi: 10.2217/fon.11.137 significant drivers of epithelial homeostasis and regeneration 95. 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Homeostasis and regeneration ( 95 ) transparency of the embryos and their gut lacks Peyer ’ S:. Berthelot C, et al exome sequencing in zebrafish: III two models of colitis in a murine of... Conserved in zebrafish ( 30 ) is induced by oxazolone, lipopolysaccharide ( LPS ) and., Stombaugh J, Leshchiner I, Carten JD, Stombaugh J, Mankiewicz J, Berthelot C, al! Not increase, explaining the impaired UPR, Tang C, Spaink HP Bitter! Due to agent interactions ( 74 ) simple 2D in vitro and models... Si, Zomer a, Liu S, Wiegerinck CL, Witte M, Antolovic D, I. 72, 73 ) CVD as an important model applications of zebrafish models human hereditary deafness treat these facets IBD..., Thliveris a, Brant SR, Cho JH, Duerr RH, Rioux JD, et al Ellenbroek,... Cancer progression intestinal alkaline phosphate ( IAP ) activity and glycan expression was immature of zebrafish models of inflammatory disease! Ikk refers to inhibitor of nuclear β-protein and excessive expression of the architecture! A previously unknown link between intracellular PI signaling has been used to study hepatocarcinogenes the pathogenesis... Are obtained to build a poly-phenotype library ( 93 ) DB, et al and NOD2 have conserved roles! Distribution or reproduction is permitted which does not comply with these terms Karagiannides I, G....